Journal: Cell reports

Article Title: The transcription factors TFEB and TFE3 link the FLCN-AMPK signaling axis to innate immune response and pathogen resistance

doi: 10.1016/j.celrep.2019.02.102

Figure Lengend Snippet: Key Resource Table

Article Snippet: Rabbit polyclonal p-ACC (S79) , Cell Signaling , Cat#3661.

Techniques: Virus, Plasmid Preparation, shRNA, Clone Assay, Recombinant, Cell Viability Assay, Gene Expression, Microarray, Over Expression, Software

Journal: International journal of molecular medicine

Article Title: LY3009120, a pan-Raf kinase inhibitor, inhibits adipogenesis of 3T3-L1 cells by controlling the expression and phosphorylation of C/EBP-α, PPAR-γ, STAT‑3, FAS, ACC, perilipin A, and AMPK.

doi: 10.3892/ijmm.2018.3890

Figure Lengend Snippet: Figure 1. Effects of LY3009120 on the adipogenesis and growth of differentiating 3T3‑L1 cells. (A) Protocol used to induce 3T3‑L1 preadipocyte differentia- tion. 3T3‑L1 preadipocytes were induced to differentiate with induction medium in the presence or absence of LY3009120 at the indicated concentrations and for the indicated times. (B) On D8, cellular lipid content was assessed by Oil Red O staining (upper panels). Phase‑contrast images of the cells were also recorded following treatment (lower panels). (C) On D8, cellular TG content was quantified using the AdipoRed assay. Values are presented as the mean ± stan- dard error of the mean of data from three independent experiments with three replicates. *P<0.05, vs. control. (D) On D8, LY3009120‑treated 3T3‑L1 cells, which are not stained by trypan blue dye, were counted under a microscope. The cell count assay was performed in triplicate. Data are presented as the mean ± standard error of the mean of data from three independent experiments. *P<0.05, vs. control. (E) 3T3‑L1 preadipocytes were induced to differentiate with induction medium in the presence or absence of LY3009120 at the indicated concentrations for 8 days. Cellular proteins were extracted and analyzed by western blot analysis. LY, LY3009120; MDI, IBMX, dexamethasone, and insulin; FBS, fetal bovine serum; D, day; TG, triglyceride; PARP, polyclonal poly (ADP‑ribose) polymerase; DR5, death receptor 5.

Article Snippet: Polyclonal p-AMPK (T172, cat. no. 2535), monoclonal AMPK (cat. no. 2793), polyclonal p-Acc (S79, cat. no. 3661), polyclonal Acc (cat. no. 3662), polyclonal liver kinase B1 (LKB1; cat. no. 3047), polyclonal p-LKB1 (S428, cat. no. 3482), polyclonal p-A-Raf (S299, cat. no. 4431), polyclonal A-Raf (cat. no. 4432), polyclonal p-B-Raf (S445, cat. no. 2696), polyclonal B-Raf (cat. no. 9433), polyclonal p-c-Raf (S259, cat. no. 9421), and monoclonal c-Raf (cat. no. 12552) antibodies were acquired from cell Signaling Technology, Inc. (danvers, MA, USA).

Techniques: Staining, Control, Microscopy, Cell Counting, Western Blot